Advancements in Human Cell Line Cryopreservation for Assay-Ready Efficiency

Cell-based bioassays are used across numerous biotechnology industries for various applications such as drug screening, vaccine development, and quality control. However, developing and deploying bioassays can be challenging due to the lengthy processes of cell culture. For instance, generating working cell banks and post-thaw recovering cryopreserved vials each require careful consideration and allocation of resources. ATCC scientists created a streamlined solution that eliminates the challenges posed by banking cells for cell-based assays. Our assay-ready cells can be used directly in cell-based assay from frozen, without the need for additional cell culture or expansion.

Here, we present application data for the ATCC Assay Ready Cells THP-1-AR (ATCC TIB-202-AR) and THP-1-NFκB-Luc2-AR (ATCC TIB-202-NFkB-LUC2-AR). Upon thaw, the cells feature high viability, fast recovery, and the ability to differentiate into macrophage-like cells that express the appropriate markers and functional attributes. These data demonstrate an assay-ready format that reduces culture time without diminishing biological relevance and has the potential to standardize and replace working cell banks for more repeatable results.

Key Points

• Generating working cell banks and post-thaw recovering cryopreserved vials presents a great challenge in the drug development process
• ATCC scientists generated a solution that allows you to go from frozen to data in one work day
• ATCC Assay Ready Cells can be used directly in cell-based assay from frozen, without the need for additional cell culture or expansion
• The thawed cells show high viability, fast recovery, and exhibit the ability to differentiate into macrophage-like cells that express the appropriate markers and functional attributes