Bifidobacterium bifidum (Tissier) Orla-Jensen

1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 mL of #2107 broth from a single test tube (5 to 6 mL) and rehydrate the entire vial contents.
3.  Aseptically transfer this aliquot back into the broth tube.  Additional tubes may be inoculated with 0.5 mL each from the suspension.  A slant and or plate may also be inoculated with 0.2 mL.  Streak a blood plate to check for purity.
4.  Incubate tubes and agar under anaerobic conditions at 37°C.  Incubate the blood plate aerobically for an aerobic contamination check.

ANAEROBIC CONDITIONS:   

Anaerobic conditions for transfer may be obtained by either of the following:

• Use of an anaerobic gas chamber, or                                         
• Placement of test tubes under a gassing cannula system connected to anaerobic gas.   

Anaerobic conditions for incubation may be obtained by any of the following:

• Loose screw caps on test tubes in anaerobic chamber,                       
• Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.  

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