Acidithiobacillus ferrooxidans (Temple and Colmer) Kelly and Wood

1. Incubate test tube cultures under above conditions upon receipt. It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth. Transfer culture to fresh media within one week of arrival.
2. Gently vortex the test-tube to dislodge the iron that has oxidized onto the glass. Aseptically withdraw approximately 1.0 mL of the broth culture and transfer into 5 mL of fresh broth.
3. Incubate the broth either on a shaker or in a static and slanted position.
4. Growth is evident within one week, when yellow-orange iron oxide deposits are observed.
5. Transfer the culture approximately every two weeks.

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