Eikenella corrodens (Eiken) Jackson and Goodman

1. Open vial according to enclosed instructions.
2. Using a single tube of #814 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant and/or plate.
5. Incubate all tubes and plate at 37°C for 24 to 72 hours.

Incubation in an atmosphere of 5% CO₂ will enhance growth.

Brain Heart Infusion Broth (BD 237500) can be used to rehydrate vial contents if #814 broth is not available, but it will not support growth as well as the GC broth.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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