Escherichia coli (Migula) Castellani and Chalmers

25922 ^™

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Escherichia coli strain Seattle 1946 is a whole-genome sequenced quality control strain that does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. It is used for media testing, as a negative control for LT toxin production, and as a quality control strain for Abbott, API, Autobac, BBL, bioMerieux VITEK, Biosynth, Difco, IDS, Micro-Media, MicroScan, Roche Diagnostics, and Sensititre products. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.

Product category: Bacteria
Product type: Preceptrol culture
Classification: Enterobacteriaceae, Escherichia
Strain designation: FDA strain Seattle 1946 [DSM 1103, NCIB 12210]
Type strain: No
Genome sequenced strain: Yes
Isolation source: Clinical isolate
Applications: Bioinformatics

Food testing

Media testing

Quality control
Product format: Freeze-dried
Storage conditions: 2°C to 8°C

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: Susceptibility disc testing cephalexin Ref. . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : .

Susceptibility disc testing cephaloglycin Ref. . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : .

Susceptibility disc testing kanamycin RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing cephaloridine cephalomycin Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref. . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : .

Food testing

Control Culture Ref Identification of Foodborne Bacterial Pathogens by Gene Probes: Enterotoxigenic Escherichia coli: Heat-Stable Enterotoxin (Human), Heat-Stable Enterotoxin (Porcine), and Heat-Labile Enterotoxin. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 24, 2001 Ref Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1681, 2005 Ref Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005

Control strain Ref Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A. Ref Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A. Ref Procedures for the Recovery and Identification of Parasites from the Intestinal Tract: Approved Guideline - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M28-A2.

Evaluation of Mueller-Hinton agar Ref Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2. RefUS General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986

Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphyloccus aureus and other species)--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 5013.12.3:2004. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003 Ref Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997. Ref Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution;British Standard DD ISO/TS 16649-3:2005. Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Quality control Ref Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

Quality control strain Ref API 20E QC Set. bioMerieux. Ref BD Crystal E/NF QC Set. BD Becton Dickinson. RefBD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson. Ref MicroScan Dried Gram Negative Panel QC Set. Beckman Coulter. RefSensititre™ Automated Gram Positive ID QC Set. Thermo Fisher Scientific. RefSensititre™ MIC Susceptibility QC Set. Thermo Fisher Scientific. RefSensititre™ Aquaculture MIC Susceptibility QC Set. Thermo Fisher Scientific.

Susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M45-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.

Susceptibility disc testing cephalothin Ref. . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : .

Susceptibility disc testing chloramphenicol RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing colistin colimycin RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing gentamicins gentamicin RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing nalidixic acid RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing neomycin RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility disc testing tetracycline RefBoyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. RefMedical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989 Ref Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994 Ref Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989 RefMedical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002 Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.

Testing Ref Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001

Quality control strain for Abbott, API, Autobac, BBL, bioMerieux VITEK, Biosynth, Difco, IDS, Micro-Media, MicroScan^®, Roche Diagnostics, and Sensititre products.

Recommended as a test microorganism for ASTM E2149; Standard Test Method for Determining the Antimicrobial Activity of Antimicrobial Agents Under Dynamic Contact Conditions
Preceptrol: Yes

Characteristics

Serotype: O6, Biotype 1
Comments: Incorrectly cited in previous editions of this catalog as identical to NCTC 10418
Biotype 1
Technical information: ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium

ATCC Medium 18: Trypticase Soy Agar/Broth

Temperature

37°C

Atmosphere

Aerobic

Handling procedure

Open vial.
Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
Incubate the tubes and plate at 37°C for 24 hours.

Handling notes

ATCC 25922 is a recommended reference strain for antibiotic susceptibility testing. It has been found that passage in broth often results in a change in MIC levels. Therefore, it is best to keep it on agar and to make stocks for storage immediately. Repeated passage is discouraged.

Purified genomic DNA of this strain is available as ATCC 25922D-5.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method: Whole-genome Sequencing

History

Deposited as: Escherichia coli (Migula) Castellani and Chalmers
Depositors: FDA
Chain of custody: ATCC <-- FDA <-- F Schoenknecht
Patent depository: This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number: 7,291,480
Cross references: GenBank AX110212 Sequence 945 from Patent WO0123604.

GenBank AX110242 Sequence 975 from Patent WO0123604.

GenBank AX111547 Sequence 2280 from Patent WO0123604.

GenBank X80724 E. coli (ATCC 25922) gene for 16S rRNA.

GenBank AF038431 Escherichia coli DNA gyrase A (gyrA) gene, partial cds.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Disclosures

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.

View All Curated Citations for this Product